Utilization of Corn Silk for GMO Detection Through Real-Time PCR

Corn silk is a fiber-rich byproduct obtained during corn processing. The present investigation deals with the purpose of GMO detection and quantification comparison DNA extraction in which considered generally waste product. A comprehensive analysis extracted quality, cost, time, PCR amplification was performed. Samples were homogenized using three different methods i.e. tissue lyser (CSK-TL) liquid nitrogen (CSK-LN), grinder (CSK-RG). samples from subjected to scanning electron microscopy (SEM) access effectiveness homogenization methods. Afterward, carried out Kits QIAGEN DNeasyMericon Food Kit, eurofins|GeneScan GENESpin isolation PENICON EZ-10 Spin Column Genomic Miniprep Kit. Furthermore, fluorometric assay done Qubit fluorometer 3.0 for DNA. quality accessed on 1.5% agarose gel. technique displays observable differences among As compared other two lyzer shows finest particle image. Whereas, images as well (CSK-RG) treated sample, show granulated particles. kits named Kit (Kit 1), 2), 3). 1 showed yield 122.4 ng/µl, whereas 2 3 gave 99.6 40.1 ng/µl respectively. By gel electrophoresis band size genomic above 1000 bp. material (corn silk) can be used through Real-Time technique. Among grinding techniques compared, Tissue lyzed sample displayed best highest quantity This method demonstrates utility products tracing status.